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human renal proximal tubular epithelial cells  (ATCC)


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    ATCC human renal proximal tubular epithelial cells
    Induction and knockdown of IFIT2 in renal tubular <t>epithelial</t> cells. (A–B) IFN‐ γ –induced IFIT2 expression in HK‐2 and RPTEC cells. (C–D) TGF‐ β 1–induced IFIT2 expression in HK‐2 and RPTEC cells. (E–F) Validation of IFIT2 knockdown efficiency by qPCR. Data are presented as mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001.
    Human Renal Proximal Tubular Epithelial Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 751 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+renal+proximal+tubule+epithelial+cells/Primary+Renal+Proximal+Tubule+Epithelial+Cells%3B+Normal%2C+Human/pmc13179815-45-0-11
    Average 99 stars, based on 751 article reviews
    human renal proximal tubular epithelial cells - by Bioz Stars, 2026-10
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    1) Product Images from "Cross‐Cohort Transcriptomic Integration Identifies IFIT2 as a Translational Diagnostic Biomarker and Functional Driver of Inflammation‐Linked Tubular Injury in Chronic Kidney Disease"

    Article Title: Cross‐Cohort Transcriptomic Integration Identifies IFIT2 as a Translational Diagnostic Biomarker and Functional Driver of Inflammation‐Linked Tubular Injury in Chronic Kidney Disease

    Journal: Human Mutation

    doi: 10.1155/humu/8282277

    Induction and knockdown of IFIT2 in renal tubular epithelial cells. (A–B) IFN‐ γ –induced IFIT2 expression in HK‐2 and RPTEC cells. (C–D) TGF‐ β 1–induced IFIT2 expression in HK‐2 and RPTEC cells. (E–F) Validation of IFIT2 knockdown efficiency by qPCR. Data are presented as mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001.
    Figure Legend Snippet: Induction and knockdown of IFIT2 in renal tubular epithelial cells. (A–B) IFN‐ γ –induced IFIT2 expression in HK‐2 and RPTEC cells. (C–D) TGF‐ β 1–induced IFIT2 expression in HK‐2 and RPTEC cells. (E–F) Validation of IFIT2 knockdown efficiency by qPCR. Data are presented as mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001.

    Techniques Used: Knockdown, Expressing, Biomarker Discovery

    IFIT2 knockdown attenuates IFN‐ γ –induced injury and apoptosis in renal tubular epithelial cells. (A–B) CCK‐8 assay showing that IFIT2 knockdown alleviates IFN‐ γ –induced reduction of cell viability in HK‐2 and RPTEC cells. (C–F) Annexin V/PI flow cytometry analysis showing that IFIT2 knockdown reduces IFN‐ γ –induced apoptosis in (C, E) HK‐2 and (D, F) RPTEC cells. Data are presented as mean ± SD from three independent experiments. ∗∗∗ p < 0.001.
    Figure Legend Snippet: IFIT2 knockdown attenuates IFN‐ γ –induced injury and apoptosis in renal tubular epithelial cells. (A–B) CCK‐8 assay showing that IFIT2 knockdown alleviates IFN‐ γ –induced reduction of cell viability in HK‐2 and RPTEC cells. (C–F) Annexin V/PI flow cytometry analysis showing that IFIT2 knockdown reduces IFN‐ γ –induced apoptosis in (C, E) HK‐2 and (D, F) RPTEC cells. Data are presented as mean ± SD from three independent experiments. ∗∗∗ p < 0.001.

    Techniques Used: Knockdown, CCK-8 Assay, Flow Cytometry

    Related Articles

    Cell Culture:

    Article Title: Cytochrome P450 and UDP-Glucuronosyltransferase Expressions, Activities, and Induction Abilities in 3D-Cultured Human Renal Proximal Tubule Epithelial Cells.
    Article Snippet: 1 Cytochrome P450 and UDP-glucuronosyltransferase expressions, activities, and 1 induction abilities in 3D-cultured human renal proximal tubule epithelial cells 2 3 Shiori Hashiba 1 , Masataka Nakano 1,2 , Itsuki Yokoseki 1 , Etsushi Takahashi 3 , Masayuki 4 Kondo 3 , Yoichi Jimbo 3 , Naoki Ishiguro 4 , Hiroshi Arakawa 5 , Tatsuki Fukami 1,2 , and Miki 5 Nakajima 1,2 6 7 1 Drug Metabolism and Toxicology, Faculty of Pharmaceutical Sciences, Kanazawa 8 University, Kakuma-machi, Kanazawa 920-1192, Japan 9 2 WPI Nano Life Science Institute (WPI-NanoLSI) Kanazawa University, Kakuma-machi, 10 Kanazawa 920-1192, Japan 11 3 R&D Department, Precision Engineering Center, Industrial Division, Nikkiso Co., Ltd., 12 Hokuyo-dai, Kanazawa 920-0177, Japan 13 4 Department of Pharmacokinetics and Nonclinical Safety, Nippon Boehringer Ingelheim Co. 14 Ltd., Chuo-ku, Kobe 650-0047, Japan 15 5 Pharmaceutical and Health Sciences, Faculty of Pharmaceutical Sciences, Kanazawa 16 University, Kakuma-machi, Kanazawa, 920-1192, Japan 17 18 at A SPE T Journals on O ctober 1, 2024 dm d.aspetjournals.org D ow nloaded from 2

    Article Title: Osmotic pressure-induced calcium response states.
    Article Snippet: .. Human umbilical vein endothelial cells (HUVEC; Lonza, Basel, Switzerland), Madin–Darby canine kidney cells (MDCK/NBL-2; Japanese Collection of Research Bioresources [JCRB], Osaka, Japan), and human renal proximal tubule epithelial cells (RPTEC/ TERT1; ATCC,Manassas, VA, USA) were cultured following the manufacturer’s instructions. .. DMEM medium (No. 043–30085; Fujifilm Wako, Osaka, Japan) with 10% fetal bovine serum (FBS) (Sigma-Aldrich, St.Louis, MO, USA) and 0.02% penicillin–streptomycin solution (Sigma-Aldrich, St. Louis, MO, USA) was used for normal cell culture.

    Article Title: Osmotic pressure‐induced calcium response states
    Article Snippet: .. Human umbilical vein endothelial cells (HUVEC; Lonza, Basel, Switzerland), Madin–Darby canine kidney cells (MDCK/NBL‐2; Japanese Collection of Research Bioresources [JCRB], Osaka, Japan), and human renal proximal tubule epithelial cells (RPTEC/TERT1; ATCC, Manassas, VA, USA) were cultured following the manufacturer's instructions. .. DMEM medium (No. 043–30085; Fujifilm Wako, Osaka, Japan) with 10% fetal bovine serum (FBS) (Sigma‐Aldrich, St.Louis, MO, USA) and 0.02% penicillin–streptomycin solution (Sigma‐Aldrich, St. Louis, MO, USA) was used for normal cell culture.

    Hood:

    Article Title: Toxicity induced by orellanine from the mushroom Cortinarius orellanus in primary renal tubular proximal epithelial cells (RPTEC): Novel mechanisms of action.
    Article Snippet: The toxicity of Orellanine (OR), a significant factor in mushroom poisoning, has severe effects on the kidneys, particularly the proximal tubules.. This study investigated the acute toxicity of OR from the Cortinarius orellanus mushroom in human Primary Renal Tubular Proximal Epithelial Cells (RPTEC).. Additionally, the half maximal inhibitory concentration (IC50) of OR in MCF-7 cells was established.



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    Induction and knockdown of IFIT2 in renal tubular epithelial cells. (A–B) IFN‐ γ –induced IFIT2 expression in HK‐2 and RPTEC cells. (C–D) TGF‐ β 1–induced IFIT2 expression in HK‐2 and RPTEC cells. (E–F) Validation of IFIT2 knockdown efficiency by qPCR. Data are presented as mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001.

    Journal: Human Mutation

    Article Title: Cross‐Cohort Transcriptomic Integration Identifies IFIT2 as a Translational Diagnostic Biomarker and Functional Driver of Inflammation‐Linked Tubular Injury in Chronic Kidney Disease

    doi: 10.1155/humu/8282277

    Figure Lengend Snippet: Induction and knockdown of IFIT2 in renal tubular epithelial cells. (A–B) IFN‐ γ –induced IFIT2 expression in HK‐2 and RPTEC cells. (C–D) TGF‐ β 1–induced IFIT2 expression in HK‐2 and RPTEC cells. (E–F) Validation of IFIT2 knockdown efficiency by qPCR. Data are presented as mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001.

    Article Snippet: Human renal proximal tubular epithelial cells, including the HK‐2 cell line (ATCC, RRID: CVCL_0302) and primary RPTEC cells (ATCC, RRID: CVCL_K278), were used in this study.

    Techniques: Knockdown, Expressing, Biomarker Discovery

    IFIT2 knockdown attenuates IFN‐ γ –induced injury and apoptosis in renal tubular epithelial cells. (A–B) CCK‐8 assay showing that IFIT2 knockdown alleviates IFN‐ γ –induced reduction of cell viability in HK‐2 and RPTEC cells. (C–F) Annexin V/PI flow cytometry analysis showing that IFIT2 knockdown reduces IFN‐ γ –induced apoptosis in (C, E) HK‐2 and (D, F) RPTEC cells. Data are presented as mean ± SD from three independent experiments. ∗∗∗ p < 0.001.

    Journal: Human Mutation

    Article Title: Cross‐Cohort Transcriptomic Integration Identifies IFIT2 as a Translational Diagnostic Biomarker and Functional Driver of Inflammation‐Linked Tubular Injury in Chronic Kidney Disease

    doi: 10.1155/humu/8282277

    Figure Lengend Snippet: IFIT2 knockdown attenuates IFN‐ γ –induced injury and apoptosis in renal tubular epithelial cells. (A–B) CCK‐8 assay showing that IFIT2 knockdown alleviates IFN‐ γ –induced reduction of cell viability in HK‐2 and RPTEC cells. (C–F) Annexin V/PI flow cytometry analysis showing that IFIT2 knockdown reduces IFN‐ γ –induced apoptosis in (C, E) HK‐2 and (D, F) RPTEC cells. Data are presented as mean ± SD from three independent experiments. ∗∗∗ p < 0.001.

    Article Snippet: Human renal proximal tubular epithelial cells, including the HK‐2 cell line (ATCC, RRID: CVCL_0302) and primary RPTEC cells (ATCC, RRID: CVCL_K278), were used in this study.

    Techniques: Knockdown, CCK-8 Assay, Flow Cytometry

    Treatment of synchronized kidney and muscle cells with recombinant Fbln5 (10ng/mL) disrupts rhythmic gene expression. A,B) Disrupted expression of Clock and Bmal1 but not C) Per2 in mouse myotube C2C12 cells with Fbln5 treatment. D,E) Disrupted expression of Bmal1 and Per1 but not F) Clock in human renal proximal tubule endothelial cells (RPTEC) with Fbln5. n=3 repetitions with biological triplicate. *p<0.05 Vehicle vs Fbln5. Cells were synchronized with dexamethasone prior to treatment with Fbln5 or vehicle.

    Journal: Comprehensive Physiology

    Article Title: The cardiac circadian clock regulates rhythms in peripheral tissues via Fibulin 5

    doi: 10.1002/cph4.70147

    Figure Lengend Snippet: Treatment of synchronized kidney and muscle cells with recombinant Fbln5 (10ng/mL) disrupts rhythmic gene expression. A,B) Disrupted expression of Clock and Bmal1 but not C) Per2 in mouse myotube C2C12 cells with Fbln5 treatment. D,E) Disrupted expression of Bmal1 and Per1 but not F) Clock in human renal proximal tubule endothelial cells (RPTEC) with Fbln5. n=3 repetitions with biological triplicate. *p<0.05 Vehicle vs Fbln5. Cells were synchronized with dexamethasone prior to treatment with Fbln5 or vehicle.

    Article Snippet: The human Renal Proximal Tubule Epithelial Cell (RPTEC) immortalized cell line (ATCC, PCS-400–010) was cultured in complete growth medium consisting of the base medium containing DMEM: F12 Medium (ATCC 30–2006) and the RPTEC Growth Kit components (ATCC ACS-4007) consisting of 5 pM triiodo-L-thyronine, 10 ng/mL recombinant human EGF, 3.5 μg/mL ascorbic acid, 5.0 μg/mL human transferrin, 5.0 μg/mL insulin, 25 ng/mL prostaglandin E 1 , 25 ng/mL hydrocortisone, 8.65 ng/mL sodium selenite and 1.2 mg/mL sodium bicarbonate.

    Techniques: Recombinant, Gene Expression, Expressing